When speaking with food safety managers trying to upgrade their testing methods, we are often asked —what is real-time PCR? How does it work? The quick answer is: PCR (or polymerase chain reaction, for those science nerds out there) is a technique for multiplying a specific strand of DNA or RNA millions of times by manipulating the cell’s natural machinery. Once multiplied or amplified, the target DNA can readily be detected though a variety of methods.
Microbial life is incredibly diverse. There are microbes that can survive the crushing pressure, low temperatures, and lack of light in the deep ocean, and others that have a thousand times more resistance to radiation than humans. A nonillion bacteria exist in the world (10 30 ), more than the number of stars in the […]
Neil Sharma All Posts, Corrosion Archaea, Bug Bottles, corrosion, IRB, Iron reducing bacteria, methanogen, MIC, microbial corrosion, Microbiologically induced Corrosion, Oil, point of need, qPCR, SRB, sulfate reducing bacteria, Water
Microbiologically Influenced Corrosion (MIC) causes billions of dollars in damage annually. It affects diverse industries including marine transportation and shipping, chemical and oil processing, power generation, water treatment, and aviation among others. But I find that most people aren’t familiar with it and I’m often asked for some specific examples. Here are two cases of MIC that we can all appreciate.
Neil Sharma All Posts, Corrosion Archaea, Beyond the Dinner Table, Bug Bottles, corrosion, IRB, Iron reducing bacteria, methanogen, MIC, Microbiologically induced Corrosion, Oil, point of need, qPCR, SRB, sulfate reducing bacteria, Water
In the past on this blog, we’ve talked a lot about using molecular testing to look for things like food borne pathogens and for verifying species ID. But point-of-need based Real-Time PCR is a powerful technology that can be used in a multitude of additional areas ranging from human health to forensics. And did you know that it can also be used in the fight against corrosion?!?